Contribution of the Pittet's index, antigen assay, IgM, and total antibodies in the diagnosis of invasive candidiasis in intensive care unit
✍ Scribed by O. Eloy; S. Marque; B. Mourvilliers; P. Pina; P.Y. Allouch; B. Pangon; J.P. Bedos; J.C. Ghnassia
- Publisher
- Masson Editeur
- Year
- 2006
- Tongue
- French
- Weight
- 223 KB
- Volume
- 16
- Category
- Article
- ISSN
- 1156-5233
No coin nor oath required. For personal study only.
✦ Synopsis
Objective. -Nosocomial invasive candidiasis is about 15% of nosocomial infections in patients in intensive care units (ICUs). Clinical signs of severe infection lack specificity, and bloodstream lack of sensitivity. Suggested tools for improving the prognosis by providing an early diagnosis include the colonization index (CI), mannan antigenemia, and anti-Candida antibodies.
Methods. -We evaluated these three tools in an ICU. Patients at risk for candidiasis were screened for colonization at ICU admission, and then once a week. The CI was determined at each timepoint. Serum was collected at the same timepoints for determination of mannan antigen (ELISA Platelia ® , Bio-Rad, France), anti-Candida IgM (ELISA, Candiquant-IgM ® , Biomerica distributed by Biotrin, Lyon, France), and total anti-Candida antibodies (immunofluorescence).
Results. -During the 2-year study period, there were 75 eligible patients. In the 46 medical patients, antigenemia had the best sensitivity and specificity (75% and 57%) but failed to sepa-