Contribution of the host to test results in assays of staphylococcus epidermidis

✍ Scribed by Betty Herndon; Lawrence Dall; Padma Suvarna

• Book ID: 102878733
• Publisher: John Wiley and Sons
• Year: 1995
• Tongue: English
• Weight: 751 KB
• Volume: 9
• Category: Article
• ISSN: 0887-8013
• DOI: 10.1002/jcla.1860090202

✦ Synopsis

From two human populations (one pediatric and one adult), clinically diagnosed with Staphylococcus epidermidis (S. epidermidis) sepsis of similar severity, bacteria were isolated from pre-antibiotic blood samples and evaluated for virulence. The LD50 of the bacteria in a mouse model was performed, with evaluation of animals dying acutely following intravenous S. epidermidis administration. More simple assays of virulence were also performed, including bacterial adherence to a fibrin clot and carbohydrate specific lectin binding. The eight pediatric-host S. epidermidis isolates required a significantly larger dose to produce lethality in dosed animals (LD50) when compared to the 20 adult-host S. epidermidis isolates.The fibrin clot assay, a test that has corroborated