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Contrasting Hydration Changes for Ethidium and Daunomycin Binding to DNA

✍ Scribed by Qu, Xiaogang; Chaires, Jonathan B.

Book ID
127081443
Publisher
American Chemical Society
Year
1999
Tongue
English
Weight
30 KB
Volume
121
Category
Article
ISSN
0002-7863

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## Abstract Calorimetric titrations have been performed on the binding of ethidium and propidium to calf thymus DNA at temperatures in the 15–60Β°C range. Enthalpy changes (Ξ”__H__~B~) derived from these experiments performed with the new Omega reaction calorimeter have a precision of Β±0.10 kcal/mol

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## Synopsis Enthalpy changes ( A H , ) for the binding of ethidium (a monocation) and propidium (a dication) to calf thymus DNA have been determined calorimetrically in piperazine-N, N'-bis(Bethanesulfonic acid) buffer with the fluoride ion as the counterion. Heats of dilution for the fluoride sal