Continuous streptomycin fermentation

✍ Scribed by Sikyta, Bohumil ;Doskočil, Jiří ;Kašparová, Jitka

• Book ID: 102875572
• Publisher: Wiley (John Wiley & Sons)
• Year: 1959
• Weight: 622 KB
• Volume: 1
• Category: Article
• ISSN: 0368-1467
• DOI: 10.1002/jbmte.390010405

✦ Synopsis

Abstract

A pilot plant unit for continuous cultivation of hyphaeforming micro‐organisms, providing for a uniform flow velocity in fermentations on suspension media with a massive growth of mycelium, is described. For the maintenance of aseptic conditions, over‐pressre of air is maintained in the whole equipment. This equipment has been used for studies of the continuous biosynthesis of streptomycin. A three‐stage fermentation proved the most advantageous. The first stage serves for multiplication of the inoculum; the second and third for the formation of the antibiotic. The system was maintained for 300–400 h with yields amounting to 2,000–2,500 u. of streptomycin/ml without any signs of contamination or degeneration of the growing culture.