Continuous spectrophotometric assay of mammalian phosphoinositide-specific phospholipase Cδ1 with a thiophosphate substrate analog
✍ Scribed by H.Stewart Hendrickson
- Book ID
- 117049704
- Publisher
- Elsevier Science
- Year
- 1998
- Tongue
- English
- Weight
- 134 KB
- Volume
- 1392
- Category
- Article
- ISSN
- 0005-2760
No coin nor oath required. For personal study only.
📜 SIMILAR VOLUMES
A thiophosphate analog of dioctanoylphosphatidylcholine has been used as the substrate in a continuous spectrophotometric assay for the Bacillus cereus phospholipase C. The reaction has been monitored at 412 nm using 5,5'-dithiobis(2-nitrobenzoic acid) (DTNB) and at 324 nm using 4,4'-dithiopuridine
A fluorescent water-soluble substrate for phosphatidylinositol-specific phospholipase C was synthesized. The diacylglycerol moiety of the natural substrate, phosphatidylinositol, was replaced by the fluorescent moiety, 2-naphthol, resulting in the synthetic substrate, racemic 2-naphthyl myo-inositol