✦ LIBER ✦

Continuous separation of proteins by poly(vinyl alcohol) shielded Multichannel Flow Electrophoresis

✍ Scribed by Zheng Liu; Jin Wang; Zheng Huang; Fuxin Ding; Zhongyao Shen; Naiju Yuan

Publisher: Springer-Verlag
Year: 1996
Tongue: English
Weight: 307 KB
Volume: 10
Category: Article
ISSN: 0951-208X
DOI: 10.1007/bf00184024

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✦ Synopsis

A hydrophilic polysulphone membrane spaced multicompartment electrolyzer for Multichannel Flow Electrophoresis (MFE) was developed. Application of poly(vinyl alcohol) (PVA) in MFE resulted in a 35% increase of protein transmembrane flux. Continuous separation of bovine serum albumin (BSA) and haemoglobin bovine blood (HBB) model mixture by PVA shielded MFE yielded 56mg BSA and 48mg HBB per hour. The average recovery was 65%.

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