Continuous production of tissue plasminogen activator from recombinant CHO cells in a depth filter perfusion system

✍ Scribed by Sang Kyo Choi; Ho Nam Chang; Duk Jae Oh

• Book ID: 104644600
• Publisher: Springer-Verlag
• Year: 1995
• Tongue: English
• Weight: 705 KB
• Volume: 9
• Category: Article
• ISSN: 0951-208X
• DOI: 10.1007/bf00152445

✦ Synopsis

A depth filter perfusion system (DFPS), equipped with a 40-pm polypropylene depth filter for cell immobilization, was used for the continuous production of tissue plasminogen activator (t-PA) from recombinant Chinese hamster ovary cells. Final cell density in the DFPS with oxygen control was 1.8x10' cells/ml of the total working volume and maximum t-PA productivity was 2.63 mg/L/day. Dissolved oxygen concentration in the filter matrix was successfully controlled by air sparging and stable operation was possible for more than 20 days.