Continuous production of large amounts of monoclonal immunoglobulins in hollow fibers using protein-free medium

✍ Scribed by F. Dhainaut; N. Bihoreau; J. L. Meterreau; J. Lirochon; R. Vincentelli; G. Mignot

• Book ID: 104747416
• Publisher: Springer
• Year: 1992
• Tongue: English
• Weight: 567 KB
• Volume: 10
• Category: Article
• ISSN: 0920-9069
• DOI: 10.1007/bf00376098

✦ Synopsis

The performance of a protein-free medium was compared in culture flasks with a serum-supplemented medium and with a serum free medium in terms of cell growth and monoclonal antibody production by a murine hybridoma. We present results of continuous production in hollow fiber culture systems using serum-free medium and protein-free medium. In protein-free medium, it has been possible to produce large quantities of monoclonal antibody with a productivity similar to that obtained in serum-free medium. After a two steps purification process, monoclonal antibodies were characterized by SDS-PAGE, High Performance Size Exclusion Chromatography and Free Solution Capillary Electrophoresis. SDS-PAGE and high performance chromatography analysis have showed that purified monoclonal antibodies produced in serum-free medium or protein-free medium were similar. Furthermore, Capillary Electrophoresis characterization revealed that both MAbs were constituted by three isoforms with equivalent electrophoretic mobilities.