Continuous production of gluconic acid and sorbitol from sucrose using invertase and an oxidoreductase of Zymomonas mobilis
β Scribed by Hyeon-Su Ro; Hak-Sung Kim
- Publisher
- Elsevier Science
- Year
- 1991
- Tongue
- English
- Weight
- 475 KB
- Volume
- 13
- Category
- Article
- ISSN
- 0141-0229
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π SIMILAR VOLUMES
A new biosensor for specific determination of sucrose was developed using an oxidoreductase of Zymomonas mobilis and invertase. Cells of Z. mobilis were permeabilized with toluene in order to utilize the enzymes of glucose-fructose oxidoreductase and gluconolactonase inside the intact cells. Permeab
For the continuous, enzymatic synthesis of sorbitol and gluconic acid by cell-free glucose-fructose oxidoreductase (GFOR) from Zymomonas mobilis, the principal determinants of productivity have been identified. Most important, the rapid inactivation of the soluble enzyme during substrate conversion