Continuous production of citric acid from sugarcane molasses using a combination of submerged immobilized and surface stabilized cultures ofAspergillus nigerKCU 520

✍ Scribed by Sanjay Gupta; Chandra B. Sharma

• Publisher: Springer Netherlands
• Year: 1994
• Tongue: English
• Weight: 343 KB
• Volume: 16
• Category: Article
• ISSN: 0141-5492
• DOI: 10.1007/bf00128607

✦ Synopsis

A high performance fermentation process for the continuous production of citric acid from sugarcane molasses by using the combination of submerged calcium alginate-immobilized and surface-stabilized cultures of Aspergillus niger KCU 520 in a continuous flow horizontal bioreactor is described. The citric acid productivity was dependent on the dilution rate with an optimum value of 0.015/h. Presaturation of fermentation medium with sterile air, in addition to surface aeration, before feeding into the bioreactor enhanced the citric acid productivity. The highest productivity, c~tr~c acid product concentration and yield obtained were 1.7 kg M-h-, ll0kg M -~ and 91% respectively. The cultures were continuously used for 30 days without any apparent loss in citric acid productivity.