Continuous production of avian sarcoma virus B77 by rat tumour cells in tissue culture

✍ Scribed by D. Šimkovič; M. Popovič; J. Švec; M. Grófová; N. Valentová

• Publisher: John Wiley and Sons
• Year: 1969
• Tongue: French
• Weight: 359 KB
• Volume: 4
• Category: Article
• ISSN: 0020-7136
• DOI: 10.1002/ijc.2910040111

✦ Synopsis

The rat tutnour RBI, previously induced by injection of fowl sarcomu B77 tissue into newborn rats, was adapted to long-term growth in tissue culture. Two cell lines, 168177 and 176177, were derived fiom the tumour. Both cell lines were capable of continuous production aiid release of infectious 877 virus over a period of more than two years' in vitro cultivation. The virus present in cell-free tissue culture media induced characteristic sarcomas upon inoculation into young chicks.

Preliminary chromosomal analysis of the uncloned population of I7BI77 cells confirmed their rat origin and revealed that the hypotriploid stem-line predoininates, being represented by 59 chromosomes.