Continuous lipolysis in a reversed micellar membrane bioreactor

✍ Scribed by D. M. F. Prazeres; F. A. P. Garcia; J. M. S. Cabral

• Book ID: 104746711
• Publisher: Springer
• Year: 1994
• Tongue: English
• Weight: 751 KB
• Volume: 10
• Category: Article
• ISSN: 1615-7605
• DOI: 10.1007/bf00373531

✦ Synopsis

The enzymatic hydrolysis of olive oil using Chromobacterium viscosum lipase B encapsulated in reversed miceltes of AOT in isooctane was carried out in a continous reversed micellar membrane bioreactor. A tubular ceramic membrane installed in an ultrafiltration module was used to retain the lipase and separate the products from the reaction media. Water filled miceUes were supplemented to the reactor together with the substrate/solvent solution to compensate for the permeation of reversed micelles. The influence of substrate concentration, residence time and water content in the productivity and conversion of the system were investigated. A linear relationship between productivity and conversion degree was found for each substrate concentration tested. Operational stability of the bioreactor was tested in a tong term operation confirming the high stability of this catalytic system.