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Continuous fluorescence microphotolysis by A sin2kx grating

✍ Scribed by Klaus Schulten

Book ID
103023191
Publisher
Elsevier Science
Year
1986
Tongue
English
Weight
524 KB
Volume
124
Category
Article
ISSN
0009-2614

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✦ Synopsis

A method is derived to evaluate the fluorescence intensity which is observed in photobleaching experiments involving a time-independent sin2kx grating. This grating corresponds to a local (position x) rate constant rsin2kx of photobleaching. Application of the method demonstrates how such experiments can yield information on lateral diffusion in artificial and biological membranes. A simple analytical approximation for the fluorescence intensity which holds for a time period less than 10/r is provided.

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✍ M. Scholz; K. Schulten; R. Peters πŸ“‚ Article πŸ“… 1985 πŸ› Springer 🌐 English βš– 679 KB

Continuous fluorescence microphotolysis (CFM) was adapted to flux measurements in single cells. The principle of the method is simple: Cells are equilibrated with a fluorescent solute, an individual cell is continuously irradiated by a laser beam focussed down to approximately the diameter of the ce