Continuous Flow Liquid Secondary Ion Mass Spectrometric Characterization of Phospholipid Molecular Species

✍ Scribed by Li, Chun; Yergey, James A.

• Publisher: John Wiley and Sons
• Year: 1997
• Tongue: English
• Weight: 595 KB
• Volume: 32
• Category: Article
• ISSN: 1076-5174
• DOI: 10.1002/(sici)1096-9888(199703)32:3<314::aid-jms494>3.0.co;2-m

✦ Synopsis

The positive and negative ion continuous Ñow liquid secondary ion mass spectrometric (CF-LSIMS) analysis of four major phospholipid classes is reported. Coupling capillary high-performance liquid chromatography with CF-LSIMS proved to be very useful for the structural identiÐcation of minor phospholipid species present in complex mixtures. Phospholipid molecular species within a particular class were separated or partially separated on the capillary column. Abundant molecular ions and characteristic fragment ions were produced. Information regarding the molecular mass, the polar head group and the fatty acyl substituents can be easily obtained, with a limit of detection below 1 ng per component.