Continuous-flow chemiluminometric determination of some tetracyclines

✍ Scribed by Aristotelis B. Syropoulos; Antony C. Calokerinos

• Book ID: 102981131
• Publisher: Elsevier Science
• Year: 1991
• Tongue: English
• Weight: 671 KB
• Volume: 255
• Category: Article
• ISSN: 0003-2670
• DOI: 10.1016/0003-2670(91)80075-5

✦ Synopsis

Reszwed 24th Apnll991) Chcmd~ce 1s found to be generated by the act1011 of lwgemn or hexacyanoferrate(III) on tetracychnes The reaction wltb haxgenm etiblts chenuhumnescence after alkahne degradation of tetracychnes to the correqondmg 180 denvat~ves The reactum wtb hexacysnoferrate(III) occurs after ac~&c degradation of tetracychnes to the correspondmg anhydro denvatwes The chemdumm~ce reactmn takes place m alkalme medmm, and allows the development of a contmuoas-flow method for the determmation of 0 100-l 20 pg ml-' tetracychne, 0 100-10 0 cg ml-' chlortetracychae and 100-10 0 cg ml-' oxytetracychne and doxycycbne When apphed to commerual formula-t~ons, the procedure was relatwely free from mterfemnces from common exupwnts The results obtamed for the assay of dosage forms compared well anth those obtamed by the official methods aad demonstrated good accuracy and preclson KeFor& Chenulummescence, Flow system, Antiblotws, Pharmaceutwals, Tetracychnes