✦ LIBER ✦

Continuous flow automated radioimmunoassay using antibodies attached to red blood cells

✍ Scribed by Stephen J. Luner

Publisher: Elsevier Science
Year: 1975
Tongue: English
Weight: 387 KB
Volume: 65
Category: Article
ISSN: 0003-2697
DOI: 10.1016/0003-2697(75)90519-9

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✦ Synopsis

Continuous-flow automated radioimmunoassays can be performed using antibodies which have been attached to red blood cells with chromic chloride. To separate the antibody-bound labeled antigen from unbound labeled antigen the erythrocytes are aggregated by Polybrene and separated from the supernatant by decantation. For the determination of serum insulin levels the method was sensitive to within 2 pU/ml when operating at a sampling rate of lO/hr with a total assay time of II/z hr.

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