Continuous enzyme processing: The isolation of prolyl-tRNA synthetase from Phaseolus Aureus

✍ Scribed by P. Dunnill; J. A. Currie; M. D. Lilly

• Publisher: John Wiley and Sons
• Year: 1970
• Tongue: English
• Weight: 545 KB
• Volume: 12
• Category: Article
• ISSN: 0006-3592
• DOI: 10.1002/bit.260120106

✦ Synopsis

A pilot-plant process has been developed for the continuous extraction and partial purification of prolyl-tRNA synthetsse from mung bean. The bean slurry was wet ground in a hammer mill, clarified by two-stage centrifugation, and the protein in the effluent fractionated by precipitation a t pH values of 5.2 and 4.2.

The improved extraction process and reduced processing time resulted in an enzyme product with a specific activity 16X that previously obtained in the batch process. The yield was also 50-60 times higher.

The throughput was 13 kg dry bean/hr.