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Continuous enzyme-coupled assay for microbial transglutaminase activity

✍ Scribed by Oteng-Pabi, Samuel K.; Keillor, Jeffrey W.

Book ID
123264767
Publisher
Elsevier Science
Year
2013
Tongue
English
Weight
410 KB
Volume
441
Category
Article
ISSN
0003-2697

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A Direct Continuous Spectrophotometric A
A Direct Continuous Spectrophotometric Assay for Transglutaminase Activity
✍ Pierre de MacΓ©do; Claudio Marrano; Jeffrey W. Keillor πŸ“‚ Article πŸ“… 2000 πŸ› Elsevier Science 🌐 English βš– 72 KB

Herein we report the development of a direct and continuous spectrophotometric method for determining transglutaminase (TGase) activity by using N,Ndimethyl-1,4-phenylenediamine (DMPDA) as a β₯-glutamyl acceptor substrate and carbobenzyloxy-Lglutamylglycine (Z-Gln-Gly) as a typical peptide β₯-glutamyl