The production of staphylocoagulase was studied with continuous cultures of various S. aureus strains in a simple salts medium supplemented with mannitol, casein hydrolysate and three vitamins. Conditions of low oxygen availability and magnesium-limitation were required for optimal steady-state staphylocoagulase production. It was demonstrated that the specific rate of staphylocoagulase production was dependent on the growth rate.
In two bovine strains, the production rate pattern was similar to that of an inducible enzyme sensitive to catabolite repression, although no specific inductor or repressor could be demonstrated. The human strain, on the other hand, produced staphylocoagulase constitutively. In all strains the specific rate of production of total extracellular protein was strictly proportional to the growth rate. The bovine strains produced 6 times more staphylocoagulase in chemostat culture as compared with batch cultures of the same organisms.
It is likely that mannitol functioned as an energy source rather than as a carbon source because it was converted for a major part to acetate and for a minor part to lactate and not to new cell material. Repression of staphylocoagulase production by mannitol, acetate or lactate was not observed. The probable nature of the regulating mechanism(s) underlying staphylocoagulase production is discussed.