## Abstract Peripheral blood lymphocytes from 32 patients with malignant melanoma were tested for cellβmediated cytotoxicity (CMC) against cultured autologous melanoma cells. Effector cells were prepared from venous blood by defibrination, gel sedimentation, nylon column filtration, and lysis of re
β¦ LIBER β¦
Continuous culture of T cells cytotoxic for autologous human leukaemia cells
β Scribed by ZARLING, JOYCE M.; BACH, FRITZ H.
- Book ID
- 109715892
- Publisher
- Nature Publishing Group
- Year
- 1979
- Tongue
- English
- Weight
- 484 KB
- Volume
- 280
- Category
- Article
- ISSN
- 0028-0836
- DOI
- 10.1038/280685a0
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## Abstract EBVβspecific cytotoxic T cells (Tc)^1^ induced __in vitro__ have continuously proliferated __in vitro__ for over 9 months. The longβterm maintenance of the Tc growth was dependent on periodic supplementation of both irrediated EBVβtransformed autologous lymphoblastoid cell line (LCL) ce