✦ LIBER ✦

Continuous colorimetric monitoring of the fructose bisphosphate aldolase reaction

✍ Scribed by Philmore Robertson Jr.; Irwin Fridovich

Publisher: Elsevier Science
Year: 1980
Tongue: English
Weight: 223 KB
Volume: 108
Category: Article
ISSN: 0003-2697
DOI: 10.1016/0003-2697(80)90594-1

No coin nor oath required. For personal study only.

✦ Synopsis

A simple method for continuous spectrophotometric assay of fructose-1,6-bisphosphate aldolase is described. The method is based on the reactivity of the product triose phosphates with cyanide to form compounds capable of the reduction of cytochrome c. At the concentrations employed, cyanide, acting catalytically, reacts with the enediol tautomer of the triose phosphates to generate the reductants, which reduce cytochrome c causing increased absorbance at 550 nm. The rate of increase in the rate of appearance of SO-nm absorbance is directly proportional to the concentration of aldolase present. The procedure is particularly useful for Class I aldolases, since the assay must be run under mildly alkaline conditions.

📜 SIMILAR VOLUMES

A selective reaction of fructose bisphos

A selective reaction of fructose bisphosphate aldolase with fluorescein isothiocyanate in chicken muscle extracts

✍ Andrew G. Gehring; John L. Ezzell; Herbert G. Lebherz 📂 Article 📅 2008 🏛 John Wiley and Sons 🌐 English ⚖ 441 KB

## Abstract The present work describes the selective covalent modification of fructose bisphosphate aldolase in crude extracts of chicken breast muscle by fluorescein 5′‐isothiocyanate (5′‐FITC) at pH 7.0 and 35°C. The modification was observed after 1 min while no other major soluble protein was l

Fructose 1,6-Bisphosphate Aldolase from

Fructose 1,6-Bisphosphate Aldolase from Staphylococcus carnosus: Overexpression, Structure Prediction, Stereoselectivity, and Application in the Synthesis of Bicyclic Sugars

✍ Maria Teresa Zannetti; Christiane Walter; Marion Knorst; Wolf-Dieter Fessner 📂 Article 📅 1999 🏛 John Wiley and Sons 🌐 English ⚖ 254 KB