Continuous cell suspension processing using magnetically stabilized fluidized beds

✍ Scribed by Brenda E. Terranova; Mark A. Burns

• Publisher: John Wiley and Sons
• Year: 1991
• Tongue: English
• Weight: 1015 KB
• Volume: 37
• Category: Article
• ISSN: 0006-3592
• DOI: 10.1002/bit.260370204

✦ Synopsis

The behavior of a cell suspension in a continuous magnetically stabilized fluidized bed (MSFB) was investigated both experimentally and theoretically. The low, constant pressure drop and fluidity of the solids phase in the MSFB allowed a continuous countercurrent separator to be constructed. The magnetic field eliminated all motion of the solids phase (nickel spheres) and produced a device similar to a packed-bed depth filter. Yeast cells were used as the suspended solids and the performance of the MSFB filter was assessed as a function of the bed height, solids velocity, cell concentration, and liquid composition. Removal rates could be adjusted by controlling the cell/support interaction and were found to be as high as 99%. A mathematical model was used to aid in understanding t h i s filtration and was found to agree qualitatively with all experimental observations. Comparison of the model with the data suggests that both celkell binding and cell shadowing are occurring.