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Continuous automated assay of α-amylase inhibitors

✍ Scribed by Luciano Vittozzi; Gino Morisi; Vittorio Silano

Publisher
John Wiley and Sons
Year
1976
Tongue
English
Weight
315 KB
Volume
27
Category
Article
ISSN
0022-5142

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✦ Synopsis

Abstract

A Technicon AutoAnalyzer was adapted to carry out a continuous assay of α‐amylase activity to detect the presence of α‐amylase inhibitors. The manifold design is based on the same sequence as the Bernfeld reducing‐sugar procedure and allows sufficient time to detect those inhibitors which act slowly. The technique can be used to assay protein amylase inhibitors from wheat at concentrations as low as 1.7 nM. It may also be used to detect amylase activity which can continue in spite of the presence of inhibitors.

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Control of wheat α-amylase using inhibit
Control of wheat α-amylase using inhibitors from cereals
✍ R. J. Henry; V G Battershell; P S Brennan; K Oono 📂 Article 📅 1992 🏛 John Wiley and Sons 🌐 English ⚖ 358 KB

## Abstract A survey of 46 varieties of cereals and related species (including 27 different species from the Poaceae) indicated the presence of a strong inhibitor of wheat α‐amylase in all seven __Hordeum__ species tested. Rye contained a lower level of inhibitor activity, but the other species con