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Mouse muscle fibres (gastrocnemius muscle) 3D reconstruction. Infrared excitation at 800nm. Nuclei are labelled with Hoechst33342 (red) and acquired in the epi-channel in the spectral range 440-480 nm. SHG (green) from myosin fibres is acquired in transmission channel. (Cover picture from: P. Bianchini, A. Diaspro, p.
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FTIR microscopic image, photograph and Raman microscopic image of colon tissue regions. The FTIR image is composed of 2 ร 2 individual images having a dimension of 170 ร 170 mm 2 each. The Raman image consist of 38 ร 38 spectra recorded with a step size of 10 mm. Colors represent cluster memberships
A nanosecond pulse of light is used to illuminate a cuvette containing an inert caged biological molecule under study. The light causes a photolabile caging group to be removed and thus the functionality of the molecule is triggered.
Pseudocolored confocal microscopy image of cryosectioned human skin visualizing the skin autofluorescence. The image was obtained by spectral acquisition and the result of an automated component extraction and linear unmixing using two components is shown as blue and green pixels. The field of view
A 3-D fluorescence lifetime rendering of an alexa-488 labelled mouse embryo. Contrast can be observed between the extrinsic label (1360 ps) and autofluorescence signal (1030 ps) excited at 485 nm.