✦ LIBER ✦

Contamination of thin sections: Some observations on the cause and elimination of ?embedding pepper?

✍ Scribed by Mollenhauer, Hilton H.

Publisher: Wiley (John Wiley & Sons)
Year: 1987
Tongue: English
Weight: 694 KB
Volume: 5
Category: Article
ISSN: 0741-0581
DOI: 10.1002/jemt.1060050106

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✦ Synopsis

A method is described for eliminating a form of contamination called embedding pepper that appears in, or on, thin sections following poststaining in lead citrate. The pepper is present most often in the matrixes of mitochondria, peroxisomes, and red blood cells. Formation of pepper can be prevented by treating the section with 0.5 N HC1 or 1.0% ethylenediaminetetraacetic acid (EDTA) for 1-5 min before staining in uranyl acetate or lead citrate.

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