Contacts of Elongation Factor G with the Small Ribosomal Subunit: Cross-Linking Approach
โ Scribed by A. V. Kubarenko; I. N. Lavrik; P. V. Sergiev; M. Heupl; M. Rodnina; W. Wintermeyer; A. A. Bogdanov; O. A. Dontsova
- Book ID
- 111579416
- Publisher
- SP MAIK Nauka/Interperiodica
- Year
- 2003
- Tongue
- English
- Weight
- 97 KB
- Volume
- 393
- Category
- Article
- ISSN
- 1607-6729
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The reagent sym-triazine trichloride is used as a bifunctional reagent to generate RNA-protein cross-links within intact ribosomal subunits from E. coli. The reaction takes place in a stepwise manner, involving substitution of one chlorine atom at 12 degrees and pH 8, and substitution of the second