Contact-dependent regulation of N-type calcium channel subunits during synaptogenesis

✍ Scribed by Bahls, Fredrick H. ;Lartius, Raj ;Trudeau, Louis-Eric ;Doyle, Robert T. ;Fang, Yu ;Witcher, Derrick ;Campbell, Kevin ;Haydon, Philip G.

• Publisher: John Wiley and Sons
• Year: 1998
• Tongue: English
• Weight: 377 KB
• Volume: 35
• Category: Article
• ISSN: 0022-3034
• DOI: 10.1002/(sici)1097-4695(199805)35:2<198::aid-neu6>3.0.co;2-#

✦ Synopsis

The developmental regulation of mained diffuse. Functional v-conotoxin GVIA-sensithe N-type calcium channel during synaptogenesis was tive channels were expressed in isolated neurons, alstudied using cultured rat hippocampal neurons to though the distribution of a 1B subunits was diffuse.

elucidate the roles of extrinsic versus intrinsic cues in

The distribution of the a 1B subunit and synaptotagmin the expression and distribution of this channel. Prior only became punctate when neuron-neuron contact to synapse formation, a 1B and b 3 subunits of the Nwas allowed. Thus, the expression of functional N-type type calcium channel were distributed diffusely calcium channels is the result of an intrinsic program throughout neurites, growth cones, and somata. As while extrinsic regulatory cues mediated by neuronsynaptogenesis proceeded, the subunit distributions neuron contact are required to control their distribubecame punctate and colocalized with the synaptic tion during synaptogenesis. ᭧ 1998 John Wiley & Sons, Inc. vesicle protein synaptotagmin. Isolated neurons were