Consumption of plant sterols reduces plasma and hepatic triglycerides and modulates the expression of lipid regulatory genes and de novo lipogenesis in C57BL/6J mice
✍ Scribed by Todd C. Rideout; Scott V. Harding; Peter J. H. Jones
- Publisher
- John Wiley and Sons
- Year
- 2010
- Tongue
- English
- Weight
- 165 KB
- Volume
- 54
- Category
- Article
- ISSN
- 1613-4125
No coin nor oath required. For personal study only.
✦ Synopsis
Abstract
To investigate emerging clinical data suggesting a triglyceride (TAG)‐lowering response to plant sterol (PS) therapy, we characterized changes in TAG metabolism in 16 C57BL/6J mice fed a basal control diet (CON) or the CON diet supplemented with 2% PS for 6 wk. PS consumption reduced (p<0.05) plasma (−28%) and hepatic (−30%) TAG concentrations compared with CON mice. PS consumption increased (p<0.05) hepatic lipogenic gene expression (sterol‐regulatory‐element‐binding protein 1c, 2.4‐fold of CON; fatty acid synthase, 6.5‐fold of CON) and de novo lipogenesis (4.51±0.72 versus 2.82±0.61%/day) compared with CON. PS consumption increased (p<0.05) fecal palmitate and stearate excretion and reduced body weight gain compared with CON mice. Although no change in the transcription of intestinal fatty acid absorptive genes was observed, peroxisome proliferator‐activated receptor α mRNA was reduced (p<0.05, 2.0‐fold of CON) in the PS‐fed mice. In conclusion, PS‐fed C57BL/6J mice showed pronounced reductions in plasma and hepatic TAG concentrations despite increases in hepatic lipogenic gene expression and de novo lipogenesis. Interference with intestinal fatty acid/TAG metabolism as suggested by increased fecal fatty acid loss and reduced weight gain may be associated with the TAG‐lowering response to PS consumption.