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Construction of up-and-down temperature-jump apparatus

✍ Scribed by Hiroshi Nakatani

Publisher: Elsevier Science
Year: 1985
Tongue: English
Weight: 281 KB
Volume: 149
Category: Article
ISSN: 0003-2697
DOI: 10.1016/0003-2697(85)90479-8

No coin nor oath required. For personal study only.

✦ Synopsis

A fast up-and-down temperature-jump apparatus whose dead time is about 60 ms was constructed. The principle of the method is to let the sample solution flow to the observation cell through a capillary in a heat-exchange chamber. Bubbling and cavitation effects in the observation cell at large up or down temperature jumps were eliminated by application of a nitrogen gas pressure of 2-5 bar. The down-temperature-jump method is especially effective for measuring temperature-induced conformational transitions of biopolymers and their assemblies. Q 1985 Academic Ress, Inc.

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