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Construction of the glucose isomerase deficient strain ofStreptomycesM1033 by homologous recombination

โœ Scribed by Chong Xu; Jun Liao; Wen Xie; Yang Cheng; Guoping Zhu; Yonghui Yang; Chenglu Chen; Limei Tao; Liwen Niu; Yuzhen Wang


Publisher
Springer
Year
2000
Tongue
English
Weight
450 KB
Volume
45
Category
Article
ISSN
1001-6538

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A general method for the construction of
โœ Kiel, J. A. K. W. ;Vossen, J. P. M. J. ;Venema, G. ๐Ÿ“‚ Article ๐Ÿ“… 1987 ๐Ÿ› Springer ๐ŸŒ English โš– 904 KB

A technique is presented by which mutations can be introduced into the Escherichia coli chromosome by gene replacement between the chromosome and a plasmid carrying the mutant gene. The segregational instability of plasmids in E. coli is used with high efficiency to isolate E. coli mutants. The meth