A shuttle cosmid vector, pANsCos1, has been constructed for Escherichia coli and filamentous fungi. This vector contains two cos sequences separated by a single XbaI restriction site. pANsCos1 allows the efficient construction of representative genomic libraries from as little as 15-20 micrograms of
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Construction of the first shuttle vectors for gene cloning and homologous recombination in Mycoplasma agalactiae
โ Scribed by Rohini Chopra-Dewasthaly; Marc Marenda; Renate Rosengarten; Wolfgang Jechlinger; Christine Citti
- Book ID
- 108131305
- Publisher
- John Wiley and Sons
- Year
- 2005
- Tongue
- English
- Weight
- 204 KB
- Volume
- 253
- Category
- Article
- ISSN
- 0378-1097
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Most conditional expression vectors designed for mammalian cells have been valuable systems for studying genes of interest by regulating their expressions. The available vectors, however, are reliable for the short-length cDNA clones and not optimal for relatively long fragments of genomic DNA or lo