Two kinds of yeast secretion vectors were constructed by site-directed mutagenesis of the invertase signal sequence and ligation of synthetic oligonucleotides coding appropriate signals. Each has a cloning site for a foreign gene preceded by a sequence encoding either the signal peptide cleavage sit
β¦ LIBER β¦
Construction of system for localization of target protein in yeast periplasm using invertase
β Scribed by Takanori Tanino; Takeshi Matsumoto; Hideki Fukuda; Akihiko Kondo
- Book ID
- 113803777
- Publisher
- Elsevier Science
- Year
- 2004
- Tongue
- English
- Weight
- 158 KB
- Volume
- 28
- Category
- Article
- ISSN
- 1381-1177
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We describe a simple and efficient one-step method to make cDNA libraries using homologous recombination in yeast. cDNA from any source, together with a linear vector, is used to transform yeast. Through homologous recombination and gap repair, the cDNA is unidirectionally incorporated into the yeas