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Construction of plasmids which lead to overproduction of yeast PHR1 photolyase in Saccharomyces cerevisiae and Escherichia coli

✍ Scribed by Sancar, Gwendolyn B.; Smith, Frances W.


Book ID
122886671
Publisher
Elsevier Science
Year
1988
Tongue
English
Weight
824 KB
Volume
64
Category
Article
ISSN
0378-1119

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The lysA gene of Escherichia coli has been cloned from a lambda transducing phage on various plasmids, present in different copy numbers in bacterial cells. Synthesis of the product of this gene, diaminopimelate (DAP)-decarboxylase, and its regulation have been studied. Expression does not follow a