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Construction of new vectors for high-level expression in actinomycetes

✍ Scribed by Christine J Rowe; Jesús Cortés; Sabine Gaisser; James Staunton; Peter F Leadlay


Book ID
117523231
Publisher
Elsevier Science
Year
1998
Tongue
English
Weight
253 KB
Volume
216
Category
Article
ISSN
0378-1119

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A system has been developed for synthesis and rapid purification of recombinant polypeptides expressed in frame with glutathione S-transferase (D. B. Smith and K. S. Johnson, 1988, Gene 67, 31-40). Expressed fusion proteins are purified from bacterial extracts by glutathione-agarose affinity chromat