New vectors for high level expression of
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David J. Hakes; Jack E. Dixon
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Article
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1992
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Elsevier Science
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English
⚖ 860 KB
A system has been developed for synthesis and rapid purification of recombinant polypeptides expressed in frame with glutathione S-transferase (D. B. Smith and K. S. Johnson, 1988, Gene 67, 31-40). Expressed fusion proteins are purified from bacterial extracts by glutathione-agarose affinity chromat