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Construction of hybrid plasmid vectors for cloning inEscherichia coli, Bacillus subtilis, and the cyanobacteriumAnacystis nidulans

โœ Scribed by Margaret L. Gallagher; William F. Burke


Publisher
Springer
Year
1987
Tongue
English
Weight
464 KB
Volume
16
Category
Article
ISSN
0343-8651

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Two plasmids were constructed consisting of the E. coli vector pACYC184 and the cyanobacterial plasmid pUC1. These recombinants, designated pUC104 and pUC105, can be transformed to E. coli K12 as well as to the cyanobacterium Anacystis nidulans R2 and in both hosts they express their antibiotic mark