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Construction of glucose-repressible yeast expression vectors

โœ Scribed by Yao, Bei; Marmur, Julius; Sollitti, Paul

Book ID
122720573
Publisher
Elsevier Science
Year
1993
Tongue
English
Weight
441 KB
Volume
137
Category
Article
ISSN
0378-1119

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A series of thiamine-repressible shuttle vectors has been constructed to allow a more efยฎcient DNA manipulation in Schizosaccharomyces pombe. These high-copy-number vectors with regulatable expression (pJR) are based on the backbone of the pREP-3X, pREP-41X and pREP-81X plasmids. The pJR vectors are