A system is described for gene disruption and replacement in Schizosaccharomyces pombe based on the homologous selectable marker, ura4, the structural gene for orotidine-5'-phosphate decarboxylase. The presence of a single copy of the wild-type gene can rescue a ura4 auxotrophic mutant. Furthermore,
✦ LIBER ✦
Construction of gene replacement vectors for Gram−bacteria using a genetically modified sacRB gene as a positve selection marker
✍ Scribed by Werner Selbitschka; Stefan Niemann; Alfred Pühler
- Publisher
- Springer
- Year
- 1993
- Tongue
- English
- Weight
- 416 KB
- Volume
- 38
- Category
- Article
- ISSN
- 1432-0614
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Recent demonstrations of loss of heterozygosity in a wide variety o f human cancers suggest that large multilocus genetic deletions (presumably including tumor suppressor genes) constitute a major class of genetic alteration in human carcinogenesis. Here we show that a bifunctional fusion gene (Hytk