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Construction of Covalent Membrane Protein Complexes and High-Throughput Selection of Membrane Mimics

✍ Scribed by Suk, Jae-Eun; Situ, Alan J.; Ulmer, Tobias S.

Book ID: 111872683
Publisher: American Chemical Society
Year: 2012
Tongue: English
Weight: 307 KB
Volume: 134
Category: Article
ISSN: 0002-7863
DOI: 10.1021/ja304247f

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## Background One of the major challenges for membrane protein structural genomics is establishing high-throughput cloning and expression screening methods to obtain enough purified protein in a homogeneous preparation for structural and functional studies. Here a series of ligation independent clo