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Construction of an artificial secYEG operon allowing high level secretion of α-amylase

✍ Scribed by Mulder, Kelly C.L.; Bandola, Joanna; Schumann, Wolfgang


Book ID
120346290
Publisher
Elsevier Science
Year
2013
Tongue
English
Weight
406 KB
Volume
89
Category
Article
ISSN
1046-5928

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A method is described for obtaining transgenic plants with a high level of expression of the introduced gene. Tobacco protoplasts were transformed with an expression construct containing a translational fusion between mature e-amylase from Bacillus licheniformis and the signal peptide of the tobacco