Construction of an Artificial Glutathione Peroxidase Active Site on Copolymer Vesicles

✍ Scribed by Yanzhen Yin; Xin Huang; Chunyan Lv; Liang Wang; Shuangjiang Yu; Quan Luo; Jiayun Xu; Junqiu Liu

• Publisher: John Wiley and Sons
• Year: 2010
• Tongue: English
• Weight: 710 KB
• Volume: 10
• Category: Article
• ISSN: 1616-5187
• DOI: 10.1002/mabi.201000179

✦ Synopsis

Abstract

To construct an efficient GPx mimic, a novel method for preparing polymer‐based vesicles carrying GPx‐active sites was developed. A series of block copolymers loaded with recognition and catalytic sites were synthesized based on polystyrene__‐block‐__poly[tri(ethylene glycol) methyl ether acrylate]s (PS‐PMEO~3~MAs). By altering the molar ratio of the functional copolymers, vesicles with GPx activity were obtained by self‐assembly of these functional copolymers through blending. The optimum GPx mimic constructed by the blending process exhibited high catalytic activity and acted as a real catalyst with typical saturation kinetics behavior. The method may be of benefit for designing other enzyme mimics and may cast a light on constructing other biologically related functional nanoparticles.
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