✦ LIBER ✦

Construction of an ∼2 Mb contig in the region around 80 cM of Arabidopsis thaliana chromosome 2

✍ Scribed by Ming Li Wang; Lu Huang; Deverie Bongard-Pierce; Stephen Belmonte; Eve Ann Zachgo; John Morris; Maureen Dolan; Howard Goodman

Publisher: John Wiley and Sons
Year: 1997
Tongue: English
Weight: 416 KB
Volume: 12
Category: Article
ISSN: 0960-7412
DOI: 10.1046/j.1365-313x.1997.d01-23.x

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✦ Synopsis

A method for construction of bacterial artificial chromosome (BAC) contigs from a yeast artifical chromosome (YAC) physical map is described. An ∼2 Mb contig, consisting of two large BAC contigs linked by a small YAC, has been assembled in the region around 80 cM of Arabidopsis thaliana chromosome 2. Clones from this contig will facilitate gene isolation in the region and can be used directly as substrates for DNA sequencing.