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Construction of Actinobacillus pleuropneumoniae-Escherichia coli shuttle vectors: expression of antibiotic-resistance genes

✍ Scribed by Susan E.H. West; Mary Jo M. Romero; Laura B. Regassa; Nicolette A. Zielinski; Rodney A. Welch

Book ID
116132815
Publisher
Elsevier Science
Year
1995
Tongue
English
Weight
527 KB
Volume
160
Category
Article
ISSN
0378-1119

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Bifunctional recombinant plasmids were constructed, comprised of the E. coli vectors pBR322, pBR325 and pACYC184 and different plasmids from Gram-positive bacteria, e.g. pBSU161-1 of B. subtilis and pUB110 and pC221 of S. aureus. The beta-lactamase (bla) gene and the chloramphenicol acetyltransferas