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Construction of a Tetracycline-inducible promoter inSchizosaccharomyces pombe

✍ Scribed by Khaschayar Faryar; Christiane Gatz

Publisher
Springer-Verlag
Year
1992
Tongue
English
Weight
786 KB
Volume
21
Category
Article
ISSN
0172-8083

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✦ Synopsis

We have developed a tightly repressed Schizosaccharomyces pombe promoter which can be efficiently induced by Tetracycline. This promoter is a derivative of the plant viral cauliflower mosaic virus 35S promoter which normally functions as a strong constitutive promoter in S. pombe. Location of three binding sites for the Tnl0-encoded Tet repressor in the vicinity of the TATA-box of the CaMV 35S promoter led to a tight repression of promoter activity in the presence of the Tet repressor protein. Up to a 400-fold induction was observed after addition of the inducer Tetracycline, which inactivates the operator-binding capacity of the repres-

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