Construction of a shuttle vector forZymomonas mobilis

✍ Scribed by Dong-Wuk Cho; Peter L. Rogers; Stephen F. Delaney

• Book ID: 104651006
• Publisher: Springer
• Year: 1989
• Tongue: English
• Weight: 422 KB
• Volume: 32
• Category: Article
• ISSN: 1432-0614
• DOI: 10.1007/bf00164822

✦ Synopsis

An Escherichia coli-Zymomonas mobilis shuttle vector was constructed from a 15.5 kb native plasmid of ZM6100 and the E. coli plasmid, pBR329. Integrative transfer of this shuttle vector from E. coli to Z. mobilis was achieved with the aid of the mobilizing plasmid, pRK2013. The shuttle vector was stable in Z. mobilis for at least 300 generations without antibiotic selection.