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Construction of a runaway vector and its use for a high-level expression of a cloned human superoxide dismutase gene

โœ Scribed by Tomio Morino; Makoto Morita; Kenji Seya; Yoshikazu Sukenaga; Kazuo Kato; Tsuneo Nakamura

Publisher
Springer
Year
1988
Tongue
English
Weight
555 KB
Volume
28
Category
Article
ISSN
1432-0614

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## Background One of the major challenges for membrane protein structural genomics is establishing high-throughput cloning and expression screening methods to obtain enough purified protein in a homogeneous preparation for structural and functional studies. Here a series of ligation independent clo