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Construction of a recF deletion mutant of Azotobacter vinelandii and its characterization

✍ Scribed by Hema Badran; Rashmi Sohoni; T.V Venkatesh; H.K Das

Book ID
109323030
Publisher
John Wiley and Sons
Year
1999
Tongue
English
Weight
244 KB
Volume
174
Category
Article
ISSN
0378-1097

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Characterization of the gene for the Fe-protein of the vanadium dependent alternative nitrogenase of Azotobacter vinelandii and construction of a Tn5 mutant
✍ Raina, Ramesh ;Reddy, M. Amarender ;Ghosal, Debabrota ;Das, H. K. πŸ“‚ Article πŸ“… 1988 πŸ› Springer 🌐 English βš– 762 KB

A sequence homologous to the conventional nifH gene has been cloned from a different region of the Azotobacter vinelandii genome. Tn5 insertions were obtained in this clone and the mutagenized plasmid was used for marker exchange with A. vinelandii strain CA12 (delta nifHDK) to obtain Tn5 mutants. T