Construction of a new interface for comprehensive supercritical fluid chromatography×reversed phase liquid chromatography (SFC×RPLC)

✍ Scribed by Isabelle François; Alberto dos Santos Pereira; Frédéric Lynen; Pat Sandra

• Publisher: John Wiley and Sons
• Year: 2008
• Tongue: English
• Weight: 911 KB
• Volume: 31
• Category: Article
• ISSN: 1615-9306
• DOI: 10.1002/jssc.200800267

✦ Synopsis

Abstract

The design of a new interface to hyphen high efficiency supercritical fluid chromatography (SFC) and fast RPLC in a comprehensive configuration is described. SFC×RPLC is a viable alternative to normal phase (NP) LC×RPLC and is characterized by high orthogonality. Compared to NPLC×RPLC an additional advantage is the expansion of supercritical carbon dioxide (CO~2~) when exposed to atmospheric pressure leading to fractions consisting of solvents that are miscible with the second dimension RPLC mobile phase. The interface consists of a two‐position/ten‐port switching valve equipped with two packed octadecyl silica (C~18~) loops for effective trapping and focusing of the analytes after elution from the SFC dimension. The addition of a water make‐up flow to the SFC effluent prior to entering the loops is of fundamental importance to efficiently focus the solutes on the C~18~ material and to reduce interferences of expanded CO~2~ gas on the second dimension separation. The features of the system are illustrated with the analysis of a lemon oil sample.