Construction of a monoclonal antibody against Igh-7a

✍ Scribed by Mitsuko Usui; Takao Hirano; Hiroaki Miyajima; Shunsaku Ando; Masashi Kurimoto; Chiharu Yamaji; Tyoku Matuhasi; Zoltan Ovary; Ko Okumura

• Publisher: Springer-Verlag
• Year: 1993
• Tongue: English
• Weight: 363 KB
• Volume: 37
• Category: Article
• ISSN: 0093-7711
• DOI: 10.1007/bf00187459

✦ Synopsis

Mouse immunoglobulin IgE allotype, IgEa and IgEb, was demonstrated by B orges and co-workers ( 19 81 ) by the use of alloantisera, and the locus was designated Igh-7. They showed that the locus controlling Igh-7 allotype is strongly linked with the Igh-1 locus, and that distribution of Igh-7b strictly follows the inheritance pattern of Igh-lb; on the other hand, Igh-7a allotype shows cross reactivity with Igh-la, Igh-1 a, Igh-le, and Igh-1 j. In the study on differences between the nucleic acid sequences of these alleles, Shinkai and co-workers (1988) found that there are 12 singlebase changes in the CH1-CH4 domains, two in CH1 and CH2 are silent, whereas two of the three in CH3 and five of the seven in CH4 result in amino acid substitutions