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Construction of a gentamicin C1a-overproducing strain of Micromonospora purpurea by inactivation of the gacD gene

✍ Scribed by Li, Dan; Li, Hao; Ni, Xianpu; Zhang, Hongyu; Xia, Huanzhang


Book ID
120937868
Publisher
Elsevier Science
Year
2013
Tongue
English
Weight
444 KB
Volume
168
Category
Article
ISSN
0944-5013

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When DNA fragments from Micromonospora purpurea (the producer of gentamicin) were cloned in Streptomyces lividans, a gentamicin-resistant strain was obtained in which the ribosomes were highly resistant both to gentamicin and to kanamycin. Reconstitution analysis revealed that such resistance result