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Construction of a Broad-Host-Range Promoter-Probing Vector and Cloning of Promoter Fragments ofXanthomonas campestris

โœ Scribed by Shu-Fen Weng; Mih-Yun Shieh; Fang-Yu Lai; Yuan-Yuan Shao; Juey-Wen Lin; Yi-Hsiung Tseng

Book ID
115579331
Publisher
Elsevier Science
Year
1996
Tongue
English
Weight
81 KB
Volume
228
Category
Article
ISSN
0006-291X

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A multipurpose broad host range cloning
A multipurpose broad host range cloning vector and its use to characterise an extracellular protease gene of Xanthomonas campestris pathovar campestris
โœ Liu, Yu-Ning ;Tang, Ji-Liang ;Clarke, Belinda R. ;Dow, J. Maxwell ;Daniels, Mich ๐Ÿ“‚ Article ๐Ÿ“… 1990 ๐Ÿ› Springer ๐ŸŒ English โš– 937 KB

A multipurpose broad host range plasmid, pIJ3200, was constructed by inserting the polylinker-containing 445 bp PvuII fragment of Bluescript M13 into the EcoRI site of the cosmid pLAFR1. Using this vector a protease gene of Xanthomonas campestris pathovar campestris, previously cloned in the recombi